Blastomyces dermatitidis: Stability studies on different yeast lysate antigens
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چکیده
منابع مشابه
Blastomyces dermatitidis: Stability studies on different yeast lysate antigens
In Trial 1, 19 lots of Blastomyces dermatitidis (T-58; Tennessee dog isolate) were assayed to determine the stability of the reagents following storage. The reactivity of the antigens, produced from 1989 to 2012 and stored at 4 ̊C, was determined by comparing antibody detection (enzyme-linked immunosorbent assay; ELISA) in 12 serum specimens from immunized rabbits. All of the 19 reagents produce...
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The systemic fungal organism, Blastomyces dermatitidis causes blastomycosis in animals and humans. This study was designed to evaluate antibody detection in 55 serial serum specimens from 9 dogs with blastomycosis using B. dermatitidis yeast lysate antigens produced from two human isolates (B5896; B5931) and two dog isolates (ERC-2; T-58) with the indirect enzyme linked immunosorbent assay (ELI...
متن کاملBlastomyces dermatitidis: Antibody Detection in Sera from Dogs with Blastomycosis with Yeast Lysate Antigens Produced from Human and Dog Isolates
Dogs are common hosts to the fungal organism Blastomyces dermatitidis, which causes the systemic disease blastomycosis. The goal of our study was to compare the reactivity of two B. dermatitidis yeast lysate antigens prepared from dog isolates (ERC-2, Wisconsin; T-58, Tennessee) and two lysate antigens prepared from human isolates (B5931 and B5896, Minnesota) against 48 serum specimens from dog...
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The systemic fungal infection, blastomycosis, which infects both humans and animals has presented a diagnostic challenge for clinicians for many years. The aim of this study was to evaluate the diagnostic sensitivity of Blastomyces dermatitidis yeast lysate antigens with respect to antibody detection in dogs with blastomycosis. Lysate antigens were prepared from B. dermatitidis isolates T-58 an...
متن کاملComparison of Detection of Antibodies with Yeast Lysate Antigens Prepared from Two Isolates of Blastomyces dermatitidis by Two Different Methods: Sensitivity and Specificity Evaluations
Blastomycosis, a systemic fungal disease, caused by the dimorphic fungus Blastomyces dermatitidis, has continually presented clinicians with concerns with regard to laboratory diagnosis and prevention. For years researchers have strived to develop antigens with a high degree of sensitivity and specificity. The purpose of this study was to gain a better understanding of how two novel yeast lysat...
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ژورنال
عنوان ژورنال: Open Journal of Immunology
سال: 2013
ISSN: 2162-450X,2162-4526
DOI: 10.4236/oji.2013.33014